Perpetuating human adult stem cells including germline pluripotent stem cells (hGPSCs) in human serum

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Categories: “Diagnostics” “Research Tools

Reference #: 2017-028

OTC Contact: Ruchika Nijhara Ph.D., MBA, Director (Directory Information | Send a Message)


The invention describes method to indefinitely expand human germline pluripotent stem cells (hgPSCs) from biopsy in proprietary cell culture medium. Cells can be transplanted for treating and/or curing diseases.

hgPSCs hold promise for use in regenerative medicine, however these cells grow very slowly making it impractical to utilize gPSCs to realize their potential in regenerative medicine, moreover, because so much time is required to expand these cells in culture, invariably large portions of the cells will begin to differentiate, rendering them unusable for further manipulation. These issues lead to the need for a method of culturing hgPSCs in a continuous manner that can promote rapid expansion, without differentiation.

Georgetown University researchers have developed a hgPSC culturing technique that not only address the issues above, but also overcomes issues of graft rejection and tissue availability. The present invention enables the use of hgPSCs from biopsy to be expanded and transplanted.



Stage of Development

Cell immortalization and expansion protocol established and reduced to practice on human pancreatic islet cells in a lab setting. Experiments to determine mode of action and further optimize protocol are underway.

Patent Status

Provisional application has been filed.


Gary I. Gallicano
Samiksha Mahapatra
Dianna Martin