Detection of Bacterial and Viral Pathogens Using Fluorescence Activated Sensing Technology (FAST System)

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Categories: “Diagnostics” “Viruses, Chronic & Infectious Diseases” “Research Tools

Reference #: 2004-002 & 2009-010

OTC Contact: Ruchika Nijhara Ph.D., MBA (Directory Information | Send a Message)


Researchers at Georgetown University have developed a sensitive method for detecting the presence of any microorganism (such as bacteria or virus) in an infection, contamination or otherwise that affect human and animal health. The method involves a real time isothermal reaction that amplifies the signal generated by a fluorescent DNA probe that binds specifically to the target organism. In cases where maximum speed and sensitivity are required, signal amplification occurs at the same time as targeted DNA amplification.



Stage of Development

Studies have demonstrated that the assay can be used to specifically detect the presence of (1) Bacillus anthracis pX01 and pX02 plasmids; (2) Escherichia coli genomic DNA; (3) Bacillus subtilis genomic DNA; (4) different Dengue serotypes in a highly sensitive single-tube multiplex assay; (5) Chlamydia trachomatis in human urine.

The initial work was funded by the Department of Defense (DoD) and focused on the development of a stand-alone instrument capable of detecting bioterror threat organisms. As a part of that work, Georgetown scientists developed probes against Bacillus anthracis that have been validated by DoD. The researchers developed and delivered to DoD a semi-automated prototype of a stand-alone detector that uses these probes.


Mark Danielsen, Eugene Davidson, Kenneth L. Dretchen, Berenice Alfonso, and Bolor Tumurpurev

Relevant Publications

Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA); Danielsen M et al; Nucleic Acids Res. 2007;35(18):e117.

Patent Status

US issued patents:
9,012,142 issued April 21, 2015
9,562,258 issued Feb, 7, 2017
10,023,905 issued July 17, 2018
10, 246,739 issued April 2, 2019