Detection of Bacterial and Viral Pathogens Using Fluorescence Activated Sensing Technology (FAST System)

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Categories:  “Diagnostics

Reference #: 2004-002 & 2009-010

OTC Contact: Ruchika Nijhara, Ph.D., MBA, CLP (Directory Information | Send a Message)


Researchers at Georgetown University have developed a sensitive method for detecting the presence of any microorganism (such as bacteria or virus) in an infection, contamination or otherwise that affect human and animal health.  The method involves the hybridization of a probe andtarget DNA to create a recognition site for a strand-specific enzyme (endonuclease) that cleaves the probe into two pieces leaving the target DNA intact. Laser-induced fluorescence coupled with capillary electrophoresis is then used to measure the probe cleavage products. The target DNA can then act as a template for fresh probe and the process of hybridization, cleavage and dissociation repeats.



Stage of Development

Studies are done to demonstrate that the assay can be used to specifically detect the presence of Bacillus anthracis pX01 and pX02 plasmids, E-Coli genomic DNA and Bacillus subtilis genomic DNA. Studies also completed to develop a Dengue virus assay which can detect Dengue serotypes in a highly sensitive single-tube multiplex assay.The initial work was funded by Department of Defense (DoD) and focused on the development of a stand-alone instrument capable of detecting bioterror threat organism. As a part of this work, Georgetown scientists developed probes against Bacillus anthracis that have been validated by DoD. Also, most recently, the researchers developed and delivered to DoD a semi-automated prototype of a stand-alone detector that uses these probes. 

Relevant Publications

Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA); Danielsen M et al; Nucleic Acids Res. 2007;35(18):e117. 


Mark Danielsen, Eugene Davidson, and Kenneth L. Dretchen

Patent Status

IP protected.