A Fast Quantitative Mass Spectrometric Analysis for 1,25-dihydroxyvitamin D3

Categories: “Diagnostics“

Reference #: 2010-004

OTC Contact: Sharon E. Pula, M.S., J.D. (Directory Information | Send a Message)

Description

A new technique has been developed for the accurate measurement of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) using mass spectrometry. The assay is specific for 1,25(OH)2D3, and it can be performed in a matter of minutes with simple sample preparation protocols. Vitamin D3 is critical to many physiological processes, and 1,25(OH)2D3 is its active metabolite. A 1,25(OH)2D¬3 deficiency can lead to organ and bone disorders, while excess 1,25(OH)2D3 is associated with digestive disorders and ischemic heart disease. Therefore, measurement of 1,25(OH)2D3 is an emerging tool in preventative medicine. Existing diagnostics are plagued by inaccurate and irreproducible results, and this new diagnostic technology can potentially overcome these issues.

Applications

The assay may be used as a clinical diagnostic for measuring 1,25(OH)2D3 from human serum samples.

Advantages

• Unlike many mass spectrometric assays, no derivitization of 1,25(OH)2D3 is necessary.
• The assay is specific for 1,25(OH)2D3, and can be completed in minutes.
• Sample preparation can be easily done by a laboratory technician.

Stage of Development

Proof of concept studies have been completed for the measurement of 1,25(OH)2D3. Quantitative assays and kits are being developed, and Georgetown is seeking a partner for further development and commercialization.

Relevant Publications

No references or resources available.

Inventor

Steven Soldin

Patent Status

U.S. Patent No. 9,201,079