Reference #: 2017-028
The invention describes method to indefinitely expand human germline pluripotent stem cells (hgPSCs)
from biopsy in proprietary cell culture medium. Cells can be transplanted for treating and/or curing
hgPSCs hold promise for use in regenerative medicine, however these cells grow very slowly making it
impractical to utilize gPSCs to realize their potential in regenerative medicine, moreover, because so much time is required to expand these cells in culture, invariably large portions of the cells will begin to
differentiate, rendering them unusable for further manipulation. These issues lead to the need for a method of culturing hgPSCs in a continuous manner that can promote rapid expansion, without differentiation.
Georgetown University researchers have developed a hgPSC culturing technique that not only address the issues above, but also overcomes issues of graft rejection and tissue availability. The present invention enables the use of hgPSCs from biopsy to be expanded and transplanted.
Stem cells and/or their differentiated cell types can be used to repopulate target diseased tissue, for
example, hgPSC-derived cardiomyocytes can be transplanted into fetal heart tissue to fuse with
beating heart tissue and replace diminished disease tissue
This technique induces hgPSC cell proliferation without requiring additional exogenous growth factors and J2 feeder system
Uses cultured cells from biopsy reducing chances of graft rejection in transplant recipients
Increases hgPSC volume and alleviates scarcity of donors
Absence of animal components improves transplantation safety
Stage of Development
Cell immortalization and expansion protocol established and reduced to practice on human pancreatic islet cells in a lab setting. Experiments to determine mode of action and further optimize protocol are underway.
Provisional application has been filed.
Gary I. Gallicano