Methods for the Detection of Analytes in a Sample
Section: For Industry
Category(ies): Other
Reference #: BUPE439501
OTC Contact: David Humphrey (Directory Information | Send a Message)
Description
Assays detecting human antigen-specific antibodies are useful but problematic due to contaminants in insufficiently pure antigen. Although some immunoassays utilize yeast or insect cells to allow for post-translational modifications and folding, the antigens do not carry mammalian or disease specific modifications. The present invention utilizes reporter-antigen fusion proteins to develop an immunoprecipitation assay that can quantitatively measure serum antibody reactivity with protein antigens. In these assays, the amount of light produced is proportional to the amount of soluble fusion protein captured by the antibody-bound beads. The assay has been used to successful detect antigen-specific antibodies in human cancer patient sera, as well as Hepatitis C, and HPV specific antibodies in patient sera.
Applications
Applications: The methods developed allow for the detection of presence and progress of infectious diseases, chronic inflammatory disease, allergic reactions, autoimmune diseases and cancer. The invention allows for quantitative detection of various analytes, including antibodies, proteins, antigens, carbohydrates, and lipids, in any body fluid where the analyte may be found. Detection of an analyte may be indicative of the presence or progress of a disease state.
- Methods are simple, highly sensitivity and allow for rapid detection of an analyte.
- Methods and kit are applicable to a range of diseases, including cancer.
- Detection is quantitative allows the method to be used for monitoring the progress of the disease or condition as well as monitoring the response to treatment.
- Scope of the present invention encompasses many analytes.
- Overcomes common problems associated with bacterial or yeast incorporating immunoassays.
Advantages
Stage of Development
Stage of Development: Assay has been successfully employed to detect antigen-specific antibodies such as Hepatitis C, HPV, and cancer specific antibodies in sera from human patients.
Relevant Publications
Relevant Publications: Burbelo et al. Rapid antibody quantification and generation of whole proteome antibody response profiles using LIPS (luciferase immunoprecipitation systems). Biochem Biophys Res Commun. 352(4):889-95, 2007.
Patent Status
U.S. Patent Application Publication 20070259336, filed on December 23, 2005.
