Rapid Sequence-Specific Detection of Nucleotide Sequences
Section: For Industry
Category(ies): Other
Reference #: DAMA422003
OTC Contact: Ruchika Nijhara Ph.D. (Directory Information | Send a Message)
Description
Researchers at Georgetown University have developed a sensitive method of identifying single- or double-stranded DNA sequences. The method involves the hybridization of a probe and target DNA to create a recognition site for a strand-specific endonuclease that cleaves the probe into two pieces leaving the target DNA intact. Laser-induced fluorescence coupled with capillary electrophoresis is then used to measure the probe cleavage products. The target DNA can then act as a template for fresh probe and the process of hybridization, cleavage and dissociation repeats.
Applications
Applications:
- SNP analysis
- Genotyping
- Identifying any nucleotide sequence. For example, in identifying microorganism contamination or infection
Advantages:
- No need to purify the DNA before amplification.
- Reaction is rapid; full cleavage of probe occurs within one minute.
- Method is amenable to automation.
- Highly specific method- based on the complete complementarity between the probe and the target DNA.
- The method can be multiplexed allowing the detection of multiple sequences (multiple genes in one organism or individual genes in multiple organisms).
- The assay could also be used to identify RNA sequences.
Advantages
Stage of Development
Stage of Development: Studies done to demonstrate that the assay can be used to specifically detect the presence of Bacillus anthracis pX01 and pX02 plasmids, E-Coli genomic DNA and Bacillus subtilis genomic DNA
Relevant Publications
Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA); Danielsen M et al; Nucleic Acids Res. 2007;35(18):e117.
Patent Status
US Patent application # 11/884,366, entitled, “Sequence-Specific Detection Of Nucleotide Sequences” filed on August 15, 2007
